Tolerance of Japanese larch to drought is modified by nitrogen and water regimes during cultivation of container seedlings

Improving drought tolerance of container seedlings of Japanese larch is of high importance to afforestation. We hypothesized that adequate nitrogen (N) and limited water supply would increase the tolerance of container seedlings to water-deficit stress, circumventing photoinhibition, by means of (i) enhanced photosynthetic capacity with higher leaf N and (ii) decreased water loss from leaves with lower biomass allocation into aboveground parts. Container seedlings of Japanese larch were grown under the treatment combinations of adequate (+ N: 300 mg N container(-1)) or limited (- N: 150 mg N container(-1)) N and adequate (+ W: daily irrigation) or limited (- W: twice-a-week irrigation) water. Then, seedlings were subjected to a progressive drought treatment. Higher leaf N was observed in container seedlings grown under + N and - W. During progressive drought, lower stomatal conductance and net photosynthetic rate were observed in leaves with higher leaf N at a given predawn leaf water potential. Furthermore, the maximum efficiency of PSII photochemistry (F-v/F-m) was lower in leaves with higher leaf N, suggesting that higher leaf N might impair intrinsic tolerance to drought at the leaf level contrary to expectations. Conversely, - N and - W seedlings with lower shoot biomass delayed soil drying as a whole-plant response via a reduction in leaf transpiration, leading to delayed photoinhibition as indicated by a decline in F-v/F-m. To circumvent stress at the initial stage of water deficit, lower leaf N via limited N regime and smaller shoot biomass driven by limited N and water regimes would be important.

Automated summary

To improve the drought tolerance of container seedlings of Japanese larch, adequate nitrogen supply and limited water supply are crucial. Higher leaf nitrogen decreases photosynthetic and photochemical efficiency, impairing drought tolerance. Conversely, lower shoot biomass and leaf nitrogen can delay soil drying and reduce photoinhibition.