Characteristics of EPS from Pseudomonas aeruginosa and Alcaligenes faecalis under Cd(II) stress: changes in chemical components and adsorption performance

EPS (extracellular polymeric substance) production is a self-protection mechanism by which microorganisms slow or eliminate adverse effects in unfavorable environments. In this study, Pseudomonas aeruginosa and Alcaligenes faecalis were selected to explore changes in EPS components, especially protein components, under stress caused by different concentrations of Cd(II). The results showed that the protein content in EPS was the highest. The two strains achieved maximum EPS production levels of 109.17 and 214.96 mg/g VSS at Cd(II) stress concentrations of 20 and 50 mg/L, which were increased by 52.07% and 409.69% compared with the levels exhibited before stress, respectively. The protein content correlated very well with data from adsorption experiments. Furthermore, FTIR, 3D-EEM, and XPS results illustrated that after Cd(II) stress, C-N, C=O/-COOH, and R-NO2- moieties were formed in substantial quantities, and the stress effects of Pseudomonas aeruginosa were significantly higher than those of Alcaligenes faecalis. The results of this study showed that addition of Cd(NO3)(2) effectively regulated the components of EPS, especially the protein content, and improved the adsorption capacity, which has application prospects for prevention and control of heavy metals.

Automated summary

EPS production is a self-protection mechanism that helps microorganisms counteract adverse effects in unfavorable environments. The study found that protein content in EPS was the highest, and Cd(II) stress significantly increased EPS production in two strains of bacteria. The stress also led to the formation of specific chemical moieties. The addition of Cd(NO3)(2) effectively regulated EPS components, especially protein content, and improved adsorption capacity.