4.7 Article

Cytotoxic and genotoxic evaluation of bisphenol S on onion root tips by Allium cepa and comet tests

Journal

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 29, Issue 59, Pages 88803-88811

Publisher

SPRINGER HEIDELBERG
DOI: 10.1007/s11356-022-21888-2

Keywords

Bisphenol S; Allium cepa assay; Mitotic index; DNA damage; Chromosomal aberrations; Endocrine receptors

Ask authors/readers for more resources

Bisphenol S (BPS) is a substitute of bisphenol A and widely used in various products. This study evaluated the cytotoxic and genotoxic effects of BPS on onion root meristematic cells. The results showed that BPS inhibited root growth, reduced mitotic index, and caused chromosomal aberrations and DNA damage.
Bisphenol S (BPS) is an analog of bisphenol A, which is used as substitute of BPA in many products like airport luggage tags, baby bottles, plastics, and epoxy resins etc. Bisphenol S can cause toxic effects in different organisms, i.e., mice, rat, zebrafish, and C.elegans, etc. Bisphenol S is also known as endocrine disruptor due to its ability to mimic the endocrine receptors. So, the aim of this study was to evaluate the cytotoxic and genotoxic effects of bisphenol S on meristematic cells present in onion root tips through Allium cepa (A.cepa) and comet tests. Root growth inhibition was evaluated by root growth inhibition assay. Mitotic index (MI) and chromosomal aberrations (CAs) were assessed by A.cepa assay. DNA damage was evaluated by comet assay. Root growth of A.cepa was inhibited due to bisphenol S. LC50 value calculated by root growth inhibition assay for bisphenol S was (2.6 +/- 0.63, 50 mu g/ml). Mitotic index was reduced, and chromosomal aberrations were observed, i.e., stickiness, polyploidy, and disturbed ana-telophase in anaphase and telophase stages of mitosis. In case of comet assay, DNA damage was increased in statistically significant manner (p <= 0.05). It was concluded that bisphenol S constitutes cytotoxic and genotoxic effects on A. cepa root meristematic cells. Moreover, it is suggested to explore more toxicity studies of bisphenol S at molecular level.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available