Fate and Transformation of 6:2 Fluorotelomer Sulfonic Acid Affected by Plant, Nutrient, Bioaugmentation, and Soil Microbiome Interactions

6:2 Fluorotelomer sulfonic acid (6:2 FTSA) is a dominant per-and poly-fluoroalkyl substance (PFAS) in aqueous film-forming foam (AFFF)-impacted soil. While its biotransformation mechanisms have been studied, the complex effects from plants, nutrients, and soil microbiome interactions on the fate and removal of 6:2 FTSA are poorly understood. This study systematically investigated the potential of phytoremediation for 6:2 FTSA byArabidopsis thalianacoupled with bioaugmentation ofRhodococcus jostiiRHA1 (designated as RHA1 hereafter) under different nutrient and micro-biome conditions. Hyperaccumulation of 6:2 FTSA, defined as tissue/ soil concentration > 10 and high translocation factor > 3, was observed in plants. However, biotransformation of 6:2 FTSA only occurred under sulfur-limited conditions. Spiking RHA1 not only enhanced the biotransformation of 6:2 FTSA in soil but also promoted plant growth. Soil microbiome analysis uncovered Rhodococcus as one of the dominant species in all RHA1-spiked soil. Different nutrients such as sulfur and carbon, bioaugmentation, and amendment of 6:2 FTSA caused significant changes in -microbial community structure. This study revealed the synergistic effects of phytoremediation and bioaugmentation on 6:2 FTSA removal. and highlighted that the fate of 6:2 FTSA was highly influced by the complex interactions of plants, nutrients, and soil microbiome.

Automated summary

The study investigated the potential of phytoremediation for 6:2 FTSA by Arabidopsis thaliana coupled with bioaugmentation of Rhodococcus jostii RHA1 under different nutrient and microbiome conditions. The research found that plant and microbial interactions under different nutrient and microbiome conditions have a significant impact on the removal of 6:2 FTSA, with factors such as hyperaccumulation and translocation by plants, as well as an increase in Rhodococcus, affecting the biotransformation and removal of 6:2 FTSA.