Uptake and Metabolization of HCH Isomers in Trees Examined over an Annual Growth Period by Compound-Specific Isotope Analysis and Enantiomer Fractionation

To understand the role of plants for natural attenuation, a field study was conducted to characterize the fate of HCH in trees over an annual growth period using compoundspecific isotope analysis and enantiomer fractionation. Stable and slightly higher (513C and (537Cl values of HCH of host soil samples compared to the muck (consisting nearly exclusively of HCH) revealed that masking isotope effects caused by the limited bioavailability may underestimate the real extent of HCH transformation in soil. In contrast, an increase of (513C and (537Cl values in trees indicated the transformation of HCH. A large variability of (513C and (537Cl values in trees over the growth period was observed, representing different transformation extents among different growth times, which is further supported by the shift of the enantiomer fraction (EF), indicating the preferential transformation of enantiomers also varied over the different growth periods. Based on dual-element isotope analysis, different predominant transformation mechanisms were observed during the growing seasons. Our observation implies that plants are acting as biological pumps driving a cycle of uptake and metabolization of HCH and refeed during littering to soil catalyzing their transformation. The changes of the transformation mechanism in different seasons have implications for phytoscreening and shed new light on phytoremediation of HCH at field sites.

Automated summary

A field study was conducted to characterize the fate of HCH in trees over an annual growth period. The results showed that plants play a crucial role in the uptake and transformation of HCH, with transformation extents varying over different growth periods.