Journal
ENVIRONMENT INTERNATIONAL
Volume 166, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envint.2022.107351
Keywords
PFOS alternatives; Hepatotoxicity; Transcriptomics; Gut microbiota; Adult zebrafish
Categories
Funding
- National Natural Science Foundation of China [41867064, 32101372, 42007377]
- Jiangxi Provincial Natural Science Foundation [20212BAB213040]
- Research Funds from Jiangxi Academy of Sciences [2020-JCQN-03]
- STS program of Chinese Academy of Sciences
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This study comprehensively investigated the chronic hepatotoxicity and molecular mechanisms of PFOS and its alternatives. The results showed that these compounds caused changes in liver histopathology and function, with F-53B exhibiting the highest toxicity. The study also revealed that the hepatotoxicity was initiated by the aberrant lipid metabolism and alterations in gut microbiome.
Perfluorooctane sulfonate (PFOS) has been reported to induce hepatotoxicity in wildlife and humans. Novel PFOS alternatives have been widely used following restrictions on PFOS, but little is known about their potential toxicity. Here, the first comprehensive investigation on the chronic hepatotoxicity and underlying molecular mechanisms of PFOS, 6:2Cl-PFESA (F-53B), and sodium p-perfluorous nonenoxybenzene sulfonate (OBS) was carried out on adult zebrafish through a histopathological examination, biochemical measurement, and multiomics analysis. PFOS and its alternatives caused changes in liver histopathology and liver function indices in the order of F-53B > PFOS > OBS, which was consistent with their concentration in the liver. In silico modeling and transcriptional profiles suggested that the aberrant hepatic lipid metabolism induced by F-53B and PFOS was initiated by the action on peroxisome proliferator-activated receptor gamma (PPAR gamma), which triggered changes in downstream genes transcription and led to an imbalance between lipid synthesis and expenditure. Gut microbiome analysis provided another novel mechanistic perspective that changes in the abundance of Legionella, Ralstonia, Brevundimonas, Alphaproteobacteria, Plesiomonas, and Hyphomicrobium might link to alterations in the PPAR pathway based on their significant correlation. This study provides insight into the molecular mechanisms of hepatotoxicity induced by PFOS and its novel alternatives and highlights the need for concern about their environmental exposure risks.
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