Journal
EMBO REPORTS
Volume 23, Issue 8, Pages -Publisher
WILEY
DOI: 10.15252/embr.202154234
Keywords
aggregation; ALS; autoinhibition; KIF5A; microtubules
Categories
Funding
- ALS association [22-PDF-605, 21-PDF-585]
- German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) Walter Benjamin Programme [492655220]
- National Institutes of Health (NIH) [R01GM098469, R01NS114636, R01NS114253, R01AG068247]
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This study confirms the association between mutations in the human KIF5A gene and ALS. The specific mutation identified leads to the production of Delta Exon27, which is neurotoxic and causes neurodegeneration and disease progression at the cellular and organism levels, ultimately leading to the development of ALS.
Mutations in the human kinesin family member 5A (KIF5A) gene were recently identified as a genetic cause of amyotrophic lateral sclerosis (ALS). Several KIF5A ALS variants cause exon 27 skipping and are predicted to produce motor proteins with an altered C-terminal tail (referred to as Delta Exon27). However, the underlying pathogenic mechanism is still unknown. Here, we confirm the expression of KIF5A mutant proteins in patient iPSC-derived motor neurons. We perform a comprehensive analysis of Delta Exon27 at the single-molecule, cellular, and organism levels. Our results show that Delta Exon27 is prone to form cytoplasmic aggregates and is neurotoxic. The mutation relieves motor autoinhibition and increases motor self-association, leading to drastically enhanced processivity on microtubules. Finally, ectopic expression of Delta Exon27 in Drosophila melanogaster causes wing defects, motor impairment, paralysis, and premature death. Our results suggest gain-of-function as an underlying disease mechanism in KIF5A-associated ALS.
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