Rapid Determination of Hydrogen Peroxide in Milk with Non-enzymatic Amperometric Sensor Based on Porous Gold Modified Screen-printed Electrode in Online Dialysis System

A rapid method for determination of hydrogen peroxide based on a non-enzymatic amperometric sensor with an online dialysis unit was developed for analysis of sample with complicated matrices. A porous gold modified gold screen-printed electrode was prepared by a simple method using 9 V-battery electrodeposition. Based on data from amperometry, a linear dynamic range can be obtained in the range of 10 to 1000 mu mol L-1, with a limit of detection of 0.37 mu mol L-1. The proposed system was successfully applied to determine H2O2 in various milk samples with rapid analysis, high electrode stability, and excellent repeatability (1.95 % RSD with 100 replications).

Automated summary

A rapid method for determining hydrogen peroxide using a non-enzymatic amperometric sensor with an online dialysis unit has been developed. This method allows for analysis of samples with complex matrices. By utilizing a porous gold modified gold screen-printed electrode, the method achieves a linear dynamic range from 10 to 1000 μmol L-1 with a limit of detection of 0.37 μmol L-1. The proposed system has been successfully applied to determine H2O2 in various milk samples, demonstrating rapid analysis, high electrode stability, and excellent repeatability (1.95% RSD with 100 replications).