Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 241, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2022.113769
Keywords
Diesel; Oil-degrading bacterial consortium; Degradation products; Community structure
Categories
Funding
- Scientific Research Foundation of Shandong University of Science and Technology [2014RCJJ015]
- scientific research fund project of National Natural Science Foundation of China [42106151]
- Project of Shandong Province Higher Educational Young Innovative Talent Introduction and Cultivation Team [Hydrogen energy chemistry innovation team]
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This study successfully isolated bacteria capable of efficiently degrading oil, established a diesel degrading bacterial consortium with good capability of degrading short chain alkanes, and found that oil pollution has a selective effect on marine microorganisms. Although oil degradation was promoted after the addition of SA, the recovery of microbial community structure took a longer time.
In the process of marine oil spill remediation, adding highly efficient oil degrading microorganisms can effectively promote oil degradation. However, in practice, the effect is far less than expected due to the inadaptability of microorganisms to the environment and their disadvantage in the competition with indigenous bacteria for nutrients. In this article, four strains of oil degrading bacteria were isolated from seawater in Jiaozhou Bay, China, where a crude oil pipeline explosion occurred seven years ago. Results of high-throughput sequencing, diesel degradation tests and surface activity tests indicated that Peseudomonas aeruginosa ZS1 was a highly efficient petroleum degrading bacterium with the ability to produce surface active substances. A diesel oil degrading bacterial consortium (named SA) was constructed by ZS1 and another oil degrading bacteria by diesel degradation test. Degradation products analysis indicated that SA has a good ability to degrade short chain alkanes, especially n-alkanes (C10-C18). Community structure analysis showed that OTUs of Alcanivorax, Peseudomona, Ruegeria, Pseudophaeobacter, Hyphomonas and Thalassospira on genus level increased after the oil spill and remained stable throughout the recovery period. Most of these enriched microorganisms were related to known alkane and hydrocarbon degraders by the previous study. However, it is the first time to report that Pseudophaeobacter was enriched by using diesel as the sole carbon source. The results also indicated that ZS1 may have a dominant position in competition with indigenous bacteria. Oil pollution has an obvious selective effect on marine microorganisms. Although the oil degradation was promoted after SA injection, the recovery of microbial community structure took a longer time.
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