Protein engineering for electrochemical biosensors

The development of electrochemical biosensors has gained tremendous attention. Protein engineering has been applied for enhancing properties of native redox enzymes, such as selectivity, sensitivity, and stability required for applicable biosensors. This review highlights recent advances of protein engineering to improve enzymatic catalysis of biosensors, facilitate electron transfer and enzyme immobilization, and construct allosteric protein biosensors. The pros and cons of different protein engineering strategies are briefly discussed, and perspectives are further provided.

Automated summary

The development of electrochemical biosensors has received significant attention, with protein engineering being used to enhance the properties of redox enzymes for applicable biosensors. This review focuses on recent advances in protein engineering for improving enzymatic catalysis, facilitating electron transfer and enzyme immobilization, and constructing allosteric protein biosensors. The pros and cons of different protein engineering strategies are briefly discussed, along with future perspectives.