Medicinal signaling cells niche in stromal vascular fraction from lipoaspirate and microfragmented counterpart

Aim To expand our previous findings by increasing the number of patients in a study characterizing medicinal signaling cells (MSC) of stromal vascular fraction from lipoaspirate (SVF-LA) and from microfragmented lipoaspirate (SVF-MLA) applied for the treatment of osteoarthritis (OA). Methods Twenty OA patients, including 8 new patients, acquiring autologous microfragmented adipose tissue were enrolled. In-parallel immunophenotyping of SVF-LA and SVF-MLA was performed. The samples were incubated in a DuraClone SC prototype tube targeting the CD31, CD34, CD45, CD73, CD90, CD105, and CD146 surface markers, stained with the DRAQ7 cell nuclear dye and Live/Dead Yellow Fixable Stain, and analyzed by flow cytometry. Results The population phenotypes in SVF-LA and SVF-MLA samples included CD31(+)CD34(+)CD73(+/-)CD90(+/-)CD105(+/-)CD146(+/-) endothelial progenitors (EP), CD31(+)CD34(-)CD73(+/-)CD90(+/-)CD105(-)CD146(+/-) mature endothelial cells, CD31(-)CD34(-)CD73(+/-)CD90(+)CD105(-)CD146(+) pericytes, CD31(-)CD34(+)CD73(+/-)CD90(+)CD105(-)CD146(+) transitional pericytes, and CD31(-)CD34(+)CD73(high)CD90(+)CD105(-)CD146(-) supra-ad ventitial-adiposestromal cells. Compared with the autologous SVF-LA samples, the prevailing cell type in SVF-MLA were EP, which outnumbered leukocytes and supra-adventitial-adipose stromal cells (SA-ASC). The ratio of progenitor cells in SVF-MLA samples differed between female and male patients, showing a higher EP-pericyte and pericyteSA-ASC ratio in men. Conclusion Our results, hallmarked by EP-enriched antiinflammatory features and indicating a possible sex-specific impact, contribute to defining the cellular composition of the clinically applied MSC serving as a regenerative cell therapy in OA.

Automated summary

This study aimed to characterize medicinal signaling cells (MSC) from adipose tissue in the treatment of osteoarthritis (OA) and expand on previous findings. The results showed that microfragmented adipose tissue had a higher population of endothelial progenitors (EP) compared to leukocytes and supra-adventitial-adipose stromal cells (SA-ASC). The ratio of progenitor cells differed between male and female patients, with men showing a higher EP-pericyte and pericyte-SA-ASC ratio.