Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 216, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2022.112601
Keywords
Metal-organic framework; Nanozyme; Peroxidase-like activity; Glucose detection
Funding
- Zhejiang Provincial Natural Science Foundation of China [LQ21H200008]
- Science and Technology Bureau of Wenzhou [Y2020108]
- National Major Infectious Disease Prevention Projects [2018ZX10201001-009]
- Key Discipline of Zhejiang Province in Medical Technology
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In this study, an artificial enzyme Cu-MOF@Pt based on nanomaterials was successfully prepared, which demonstrated its selective detection ability for glucose. This method has broad application prospects.
As an alternative to natural enzymes, artificial enzymes based on nanomaterials have attracted a lot of attention owing to their outstanding catalytic activity and high stability as well as low cost. Cu-MOF loaded with platinum nanoparticles (labeled Cu-MOF@Pt) was prepared by simple one-step wrapping method using platinum nanoparticles, copper nitrate trihydrate and 1,3,5-tricarboxybenzene. It was confirmed that Cu-MOF@Pt exhibits peroxidase-like activity, which can quickly catalyze the oxidation of 3,3 ',5,5 '-tetramethylbenzidine (TMB) and produce blue oxidized TMB (oxTMB) in the presence of hydrogen peroxide (H2O2). Additionally, steady-state kinetics showed that Cu-MOF@Pt exhibits stronger appetency to TMB and H2O2 compared with horseradish peroxidase. Thanks to the peroxidase-like activity of Cu-MOF@Pt, a highly selective colorimetric method for glucose detection has been successfully established, the linear range is 2-15 mM (R2 =0.9999) and the Limit of Detection (LOD) is 0.42 mM, with a detection range that meets clinical needs. Moreover, its good intra- and interassay precision and excellent stability make the results of glucose detection very reproducible. The detection performance of 90.09% was still maintained at 4 celcius for 2 months. In conclusion, a new nanocomposite was successfully prepared and its selective detection ability for glucose was proved, which established a good basis for the clinical development of new enzymes for biosensors.
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