Journal
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
Volume 647, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfa.2022.129031
Keywords
Cobalt phosphate; Colorimetric analysis; Hydrogen peroxide; Peroxidase-like activity
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Funding
- National Key Research and Devel-opment Plan of China [2021YFC2103300]
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The Co3(PO4)2.8 H2O nanozyme was synthesized through direct reaction between cobalt (II) and phosphate, and its peroxidase-like activity in the presence of hydrogen peroxide (H2O2) was studied. The nanozyme showed a higher H2O2 affinity and has potential applications.
The Co3(PO4)2.8 H2O nanozyme was in situ synthesized through direct reaction between cobalt (II) and phosphate, and its peroxidase-like activity was studied in the presence of hydrogen peroxide (H2O2) using a chromogenic substrate 3, 3 ', 5, 5 ' - tetramethylbenzidine (TMB). The effects of pH value, temperature, and incubation time on the conversion of TMB to oxidized TMB (oxTMB) catalyzed by Co3(PO4)2.8 H2O in the presence of H2O2 were optimized. The peroxidase-like activity of Co3(PO4)2.8 H2O conforms to the Michaelis-Menten kinetics, and its Michaelis constant (Km) is 0.073 mM, showing a higher H2O2 affinity than natural horseradish peroxidase (HRP) and some other peroxidase nanozymes. The mechanism exploration experiment confirmed that the hydroxyl radical (center dot OH) is the main active species in the catalytic reaction of Co3(PO4)2.8 H2O. Finally, based on the inherent peroxidase-like activity of Co3(PO4)2.8 H2O, a simple colorimetric detection method for H2O2 was established, with a linear range of 15.0 - 100.0 mu M and a detection limit of 4.385 mu M. The method was applied to the determination of H2O2 in milk samples with the spiked recovery of 90.2%- 106.2%. This study reports a novel nanozyme that can be easily produced in situ, which is exempt from the harsh, cumbersome, and timeconsuming synthesis and purification procedures. In addition, the H2O2 colorimetric detection method based on the nanozyme has the advantages of low cost, convenient, high sensitivity, and good selectivity.
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