Journal
CHEMISTRY-AN ASIAN JOURNAL
Volume 17, Issue 16, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.202200426
Keywords
hydrogen sulfide; fluorescent probe; cell trappable; reactive sulfur species; activity based probe
Categories
Funding
- NIH [R01GM113030]
- NSF [CHE-1531189]
Ask authors/readers for more resources
A new fluorescent probe CT-MeRhoAz has been developed for the detection of H2S. The probe shows high selectivity and cell-trapping ability for H2S, and exhibits a significantly enhanced fluorescent signal in the presence of H2S.
Hydrogen sulfide is a biologically important molecule and developing chemical tools that enable further investigations into the functions of H2S is essential. Fluorescent turn-on H2S probes have been developed for use in cellulo and in vivo, but the membrane permeability of these probes can lead to probe leakage and signal attenuation over time. Here we report a cell trappable fluorescent probe for H2S, CT-MeRhoAz, which is based on a methylrhodolazide scaffold derivatized with an acetoxymethyl ester group. Prior to ester cleavage, the CT-MeRhoAz probe generates a 2500-fold turn-on response to H2S, which is enhanced to a 3000-fold response for the carboxylic acid form of the probe. Additionally, the probe is highly selective for H2S over other biologically relevant sulfur, oxygen, and nitrogen-based analytes. Live cell imaging experiments confirmed the biocompatibility of CT-MeRhoAz and also that it is cell trappable, unlike the parent MeRhoAz scaffold.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available