Journal
CELL PROLIFERATION
Volume 55, Issue 11, Pages -Publisher
WILEY
DOI: 10.1111/cpr.13314
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Funding
- National Key Research and Development Program of China Project [2021YFA1101402/2018YFA0108001]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA16010300/XDA16021400]
- National Science Foundation of China [31900690]
- Chinese Academy of Sciences
- National Key Research and Development Program of China
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In this study, we identified ARID1A as a central regulator of microglia polarization and established a mechanistic link between chromatin remodeling, neurogenesis, and mouse behaviors, highlighting the potential development of innovative therapeutics exploiting the innate regenerative capacity of the nervous system.
Objective Microglia, the prototypical innate immune cells of the central nervous system (CNS), are highly plastic and assume their phenotypes dependent on intrinsically genetic, epigenetic regulation or extrinsically microenvironmental cues. Microglia has been recognized as key regulators of neural stem/progenitor cells (NSPCs) and brain functions. Chromatin accessibility is implicated in immune cell development and functional regulation. However, it is still unknown whether and how chromatin remodelling regulates the phenotypic plasticity of microglia and exerts what kind of effects on NSPCs. Methods We investigated the role of chromatin accessibility in microglia by deleting chromatin remodelling gene Arid1a using microglia-specific Cx3cr1-cre and Cx3cr1-CreERT2 mice. RNA-seq and ATAC-seq were performed to dissect the molecular mechanisms. In addition, we examined postnatal M1/M2 microglia polarization and analysed neuronal differentiation of NSPCs. Finally, we tested the effects of microglial Arid1a deletion on mouse behaviours. Results Increased chromatin accessibility upon Arid1a ablation resulted in enhanced M1 microglial polarization and weakened M2 polarization, which led to abnormal neurogenesis and anxiety-like behaviours. Switching the polarization state under IL4 stimulation could rescue abnormal neurogenesis, supporting an essential role for chromatin remodeler ARID1A in balancing microglial polarization and brain functions. Conclusions Our study identifies ARID1A as a central regulator of microglia polarization, establishing a mechanistic link between chromatin remodelling, neurogenesis and mouse behaviours, and highlights the potential development of innovative therapeutics exploiting the innate regenerative capacity of the nervous system.
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