Platelet-Mediated Transfer of Cardioprotection by Remote Ischemic Conditioning and Its Abrogation by Aspirin But Not by Ticagrelor

Purpose The role of platelets during myocardial ischemia/reperfusion (I/R) is ambivalent. They contribute to injury but also to cardioprotection. Repeated blood flow restriction and reperfusion in a tissue/organ remote from the heart (remote ischemic conditioning, RIC) reduce myocardial I/R injury and attenuate platelet activation. Whether or not platelets mediate RIC's cardioprotective signal is currently unclear. Methods and Results Venous blood from healthy volunteers (without or with pretreatment of 500/1000 mg aspirin or 180 mg ticagrelor orally, 2-3 h before the study, n = 18 each) was collected before and after RIC (3 x 5 min blood pressure cuff inflation at 200 mmHg on the left upper arm/5 min deflation). Washed platelets were isolated. Platelet-poor plasma was used to prepare plasma-dialysates. Platelets (25 x 10(3)/mu L) or plasma-dialysates (1:10) prepared before and after RIC from untreated versus aspirin- or ticagrelor-pretreated volunteers, respectively, were infused into isolated buffer-perfused rat hearts. Hearts were subjected to global 30 min/120 min I/R. Infarct size was stained. Infarct size was less with infusion of platelets/plasmadialysate after RIC (18 +/- 7%/23 +/- 9% of ventricular mass) than with platelets/plasma-dialysate before RIC (34 +/- 7%/33 +/- 8%). Aspirin pretreatment abrogated the transfer of RIC's cardioprotection by platelets (after/before RIC, 34 +/- 7%/33 +/- 7%) but only attenuated that by plasma-dialysate (after/before RIC, 26 +/- 8%/32 +/- 5%). Ticagrelor pretreatment induced an in vivo formation of cardioprotective factor(s) per se (platelets/plasma-dialysate before RIC, 26 +/- 7%/26 +/- 7%) but did not impact on RIC's cardioprotection by platelets/plasma-dialysate (20 +/- 7%/21 +/- 5%). Conclusion Platelets serve as carriers for RIC's cardioprotective signal through an aspirin-sensitive and thus cyclooxygenase-dependent mechanism. The P2Y(12) inhibitor ticagrelor per se induces a humoral cardioprotective signal.

Automated summary

The role of platelets during myocardial ischemia/reperfusion (I/R) is ambivalent. Remote ischemic conditioning (RIC) reduces myocardial I/R injury and platelet activation. This study found that platelets serve as carriers for RIC's cardioprotective signal and aspirin-sensitive mechanism plays a role in this process.