Journal
CARBOHYDRATE POLYMERS
Volume 287, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2022.119328
Keywords
Bioink; 3D printing; Glycol chitosan; Bone regeneration
Categories
Funding
- Ministry of Trade, Industry and Energy (MOTIE) , Republic of Korea [10047811]
- Ministry of SMEs and Startups, Republic of Korea [S2782460]
- Korea Evaluation Institute of Industrial Technology (KEIT) [10047811] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Korea Technology & Information Promotion Agency for SMEs (TIPA) [S2782460] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Although chitosan's poor water solubility limits its general printing process, an alternative called water-soluble methacrylated glycol chitosan (MeGC) has been developed for 3D printing using a visible light curing system. The MeGC-based bioink shows great potential for patient-specific scaffolds in bone regeneration.
Although chitosan is the second most abundant natural polymer on earth, with a wide range of biomaterial applications, its poor water solubility limits general printing process. We selected water-soluble methacrylated glycol chitosan (MeGC) as an alternative and prepared a MeGC-based MG-63 cell-laden bioink for 3D printing using a visible light curing system. Optimal cell-laden 3D printing of MeGC was completed at 3% using 12 mu M of riboflavin as a photoinitiator under an irradiation for 70 s, a 26-gauge nozzle, a pneumatic pressure of 120 kPa, and a printing speed of 6 mm/s, as confirmed by printability, protein adsorption, cell viability, cell proliferation, and osteogenic capability. In addition, in vitro tests showed that MeGC-70 has a viability above 92%, a prolif-eration above 96%, and a hemolysis level below 2%. The results demonstrate the potential for MeGC-70 bioinks and 3D printed scaffolds to be used as patient-specific scaffolds for bone regeneration purposes.
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