Jurkat T Cells are Immunophenotypically Distinct from T-Cell Acute Lymphoblastic Leukemia Cells Due to High-Level Surface Expression of CD5

Human leukemic T cells show decreased surface CD5 (sCD5) and increased cytoplasmic CD5 (cCD5). When we examined their expressions in the Jurkat T cells, it showed increased sCD5 and decreased cCD5, which is in sharp contrast with the pattern of CD5 expression observed for human leukemic T cells. Furthermore, this opposite pattern was due to the absence of an exonal switch between E1A and E1B. This study suggests that Jurkat cell does not retain all characteristics of T-ALL cells; thus, we should carefully interpret the data obtained using Jurkat T cell as a model cell line of T-ALL.

Automated summary

Human leukemic T cells and Jurkat T cells show opposite patterns of CD5 expression, which is due to the absence of an exonal switch between E1A and E1B. Jurkat cells do not retain all characteristics of T-ALL cells, suggesting caution when interpreting data obtained using Jurkat T cells as a model cell line of T-ALL.