Laboratory development of subaerial biofilms commonly found on buildings. A methodological review

Study of the biodeterioration of building materials is often hampered by the inaccessibility of the colonization and impossibility of sampling in protected buildings considered part of the cultural heritage. There is therefore a recognised need to develop a laboratory mesocosm that realistically represents nature and enables the study and analysis of both the substrate and the organisms involved. Although many studies have investigated the for-mation of biofilms on various building materials, there is a lack of homogeneity and consensus in the methods and protocols used, which hampers comparison of the results obtained. This review aims to identify the different methods reported in the scientific literature, to organise these according to different factors (water access and types of organism and substrata used) and thus provide the research community with a guide for selecting the most appropriate methods according to the different objectives.

Automated summary

The study of biodeterioration of building materials is hindered by the difficulty of accessing and sampling protected buildings. Therefore, there is a recognized need to develop a laboratory mesocosm that accurately represents nature and allows for the study and analysis of both the substrate and the organisms involved. Currently, there is a lack of consensus in the methods and protocols used in studying the formation of biofilms on building materials, which makes it difficult to compare results. This review aims to identify different methods reported in the scientific literature and organize them according to different factors, providing a guide for selecting the most appropriate methods based on different objectives.