Dual-emitting Iridium nanorods combining dual-regulating coreaction accelerator Ag nanoparticles for electrochemiluminescence ratio determination of amyloid-β oligomers

Iridium(III) complexes have been developed as eminent electrochemiluminescence (ECL) luminophores, but their current applications are only limited to anodic ECL emission because of weak cathodic ECL emission. This work explored poly(styrene-co-maleicanhydride) (PSMA) as functional reagent to modulate iridium(III) complexes to simultaneously emit bipolar ECL signals. The prepared iridium(III) nanorods (Ir NRs) were detected strong bipolar ECL emissions at +0.9 V and -2.0 V with N,N-diisopropylethylenediamine (DPEA) and persulfate (S2O82-) as coreactant, respectively. Meanwhile, Ag nanoparticles (Ag NPs) were developed as dual-regulating coreaction accelerator to boost the bipolar emissions of Ir NRs simultaneously. The dual-emitting Ir NRs coupled with dual-regulating coreaction accelerator Ag NPs facilitated the construction of mono-luminophore-based ECL ratio strategy for detecting amyloid-beta oligomers (A beta O). When the target A beta O appeared, the Mg2+-dependent DNAzyme-powered biped walkers were unlocked to cleave single-stranded S1 immobilized on the surface of magnetic beads (MBs), resulting in the production of massive single-stranded ST. Then, the output ST cleaved hairpin H1 captured by Ir NRs modified electrode to produce numerous single strands, which could initiate the hybridization chain reaction (HCR) between Ag NPs-labeled H2 and Ag NPs-labeled H3 to introduce abundant Ag NPs onto the electrode surface. Due to the enhancement effect of Ag NPs on the bipolar ECL emissions from Ir NRs, the ECL ratio detection of A beta O was achieved with the detection limit of 0.62 pM. The unique dual-emitting properties of Ir NRs coupled with dual-regulating effect of Ag NPs provided an interesting mono-luminophore-based ECL ratio sensing platform for biological analysis.

Automated summary

This study explores the use of poly(styrene-co-maleic anhydride) as a functional reagent to modulate iridium(III) complexes for simultaneous bipolar ECL signals. It also utilizes silver nanoparticles as dual-regulating coreaction accelerators to enhance the bipolar emissions of iridium(III) nanorods. These unique properties provide an interesting mono-luminophore-based ECL ratio sensing platform for biological analysis.