Journal
BIOELECTROCHEMISTRY
Volume 146, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2022.108135
Keywords
Azacytidine; DNA; Interaction; Voltammetry; Spectroscopy; Binding
Funding
- Turkey scholarships
Ask authors/readers for more resources
This study examines the interaction between the anti-leukemic drug azacytidine and DNA, showing that azacytidine can bind to DNA via groove binding, based on electrochemical, spectroscopic, and molecular docking studies.
This study examines the interaction between pyrimidine nucleoside analogue azacytidine, an anti-leukemic drug, and DNA by employing electrochemical, UV-vis spectroscopy, fluorescence spectroscopy and molecular docking techniques. In the electrochemical technique, azacytidine and dsDNA interaction was investigated in two different ways: (1) in solution and (2) with a biosensor using differential pulse voltammetry (DPV) at a glassy carbon electrode. The interaction between azacytidine and dsDNA at increasing interaction times was investigated in line with the changes in adenine and guanine oxidation signals. In addition, interaction studies of polyguanine-azacytidine and polyadenine-azacytidine were performed with DPV. The binding constant values were calculated as 2.420 x 10(4) M-1 and 3.266 x 10(4) M-1 at 25 degrees C using UV and fluorescence spectroscopy, respectively. In conclusion, based on electrochemical and spectroscopic methods as well as molecular docking studies, it was predicted that azacytidine can bind to dsDNA via groove binding.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available