Journal
BIOELECTROCHEMISTRY
Volume 146, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2022.108150
Keywords
Electrochemiluminescence; Ruthenium luminophore; miRNA; Electrochemical biosensor; Epilepsy biomarker
Funding
- Science Foundation Ireland (SFI) [16/RC/3948]
- European Regional Development Fund
- FutureNeuro industry partners
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This study reports the detection of a key biomarker in epilepsy, miR-134, using an environmentally sensitive electrochemiluminescent luminophore, [Ru(DPPZ)(2)PIC](2+). The ability of the sensor to quantify miR-134 in unprocessed plasma samples from healthy volunteers and people with epilepsy is demonstrated.
The detection of a key biomarker in epilepsy, miR-134, using an environmentally sensitive electrochemiluminescent luminophore, [Ru(DPPZ)(2) PIC](2+), is reported, DPPZ is dipyrido[3,2-a:2 ',3 '-c]phenazine) and PIC is (2,2 '-bipyridyl)-2(4-carboxy phenyl) imidazo [4,5][1,10] phenanthroline. A thiolated capture strand is first labelled with [Ru(DPPZ)2 PIC](2+) and then adsorbed onto a gold electrode. No significant electrochemiluminescence, ECL, is observed for immobilised Ru-labelled capture strands which is consistent with the light-switch dye being exposed to the aqueous solution. In sharp contrast, binding of the target turns on ECL. The ECL intensity, I-ECL, depends on the number of adenine spacer bases between the end of the capture sequence and the dye. The ECL intensity for the optimised system increases linearly with increasing miR-134 concentration from 100 nM to approximately 20 mu M. Single and double base mismatches produce I-ECL that are only approximately 30% and 8% respectively of that observed for the fully complementary target reflecting differences in their association constants. Significantly, the presence of BSA protein causes I-ECL to increase by less 5% in either the single or duplex circumstances. Finally, the ability of the sensor to quantify miR-134 in unprocessed plasma samples from healthy volunteers and people with epilepsy is reported.
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