Journal
BIOCONJUGATE CHEMISTRY
Volume 33, Issue 12, Pages 2262-2268Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.2c00242
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Funding
- National Institutes of Health [R35GM137987]
- National Science Foundation [1944875]
- 3M Non-Tenured Faculty Award (3M Company)
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1944875] Funding Source: National Science Foundation
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Certain supramolecular motifs, such as Cucurbit[7]uril (CB[7]) macrocycles, exhibit affinities comparable to well-known biological interactions. By modifying cells with CB[7] constructed from a polyamidoamine (PAMAM) dendrimer scaffold, guest-linked drugs can be delivered to the cell membrane, offering potential applications in biomedicine. This approach has the potential to be used for in situ imaging, modulation of cell-based therapies, or the construction of synthetic supramolecular recognition axis on the cell membrane.
ABSTRACT: The affinity possible from certain supramolecular motifs rivals that for some of the best-recognized interactions in biology. Cucurbit[7]uril (CB[7]) macrocycles, for example, are capable of achieving affinities in their binding to certain guests that rival that of biotin-avidin. Supramolecular host-guest recognition between CB[7] and certain guests has been demonstrated to spatially localize guest-linked agents to desired sites in vivo, offering opportunities to better exploit this affinity axis for applications in biomedicine. Herein, architectures of CB[7] are prepared from a polyamidoamine (PAMAM) dendrimer scaffold, installing a PEGlinked cholesterol anchor on the opposite end of the dendron to facilitate cell membrane integration. Cells are then modified with this dendritic CB[7] construct in vitro, demonstrating the ability to deliver a model guest-linked agent to the cell membrane. This approach to realize synthetic supramolecular membrane receptors may be leveraged in the future for in situ imaging or modulation of cell-based therapies or to facilitate a synthetic supramolecular recognition axis on the cell membrane.
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