Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Volume 1868, Issue 7, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bbadis.2022.166384
Keywords
Clusterin; Aggregation-prone regions; Amyloid; Amyloid-beta; Amyloid inhibitors; Alzheimer's disease
Funding
- European Union
- Greek national funds through the Operational Program Competitiveness, Entrepreneurship and Innovation, under the call RESEARCH - CREATE - INNOVATE [T1EDK-00353]
- project INSPIRED-The National Research Infrastructures on Integrated Structural Biology, Drug Screening Efforts and Drug target functional characterization [MIS 5002550]
- Operational Program Competitiveness, Entrepreneurship and Innovation [NSRF 2014-2020]
- European Union (European Regional Development Fund)
- Greek Research & Technology Network (GRNET) at the National HPC facility - ARIS [PR007003-AbetaDynamics]
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Clusterin is identified as a glycoprotein involved in amyloid formation and has aggregation-prone regions that can form amyloid-like fibrils while also inhibiting amyloid-beta fibril formation. These findings suggest a potential role of clusterin in the molecular mechanism of inhibiting amyloid formation and indicate a possible involvement of molecular chaperones with amyloidogenic properties in regulating amyloid formation.
Clusterin is a heterodimeric glycoprotein (alpha- and beta-chain), which has been described as an extracellular mo-lecular chaperone. In humans, clusterin is an amyloid-associated protein, co-localizing with fibrillar deposits in several amyloidoses, including Alzheimer's disease. To clarify its potential implication in amyloid formation, we located aggregation-prone regions within the sequence of clusterin alpha-chain, via computational methods. We had peptide-analogues, which correspond to each of these regions, chemically synthesized and experimentally demonstrated that all of them can form amyloid-like fibrils. We also provide evidence that the same peptide-analogues can inhibit amyloid-beta fibril formation, potentially making them appropriate drug candidates for Alzheimer's disease. At the same time, our findings hint that the respective aggregation-prone clusterin regions may be implicated in the molecular mechanism in which clusterin inhibits amyloid formation. Furthermore, we suggest that molecular chaperones with amyloidogenic properties might have a role in the regulation of amyloid formation, essentially acting as functional amyloids.
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