Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 618, Issue -, Pages 73-78Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2022.06.017
Keywords
Poly(A) tail; mRNA; Translation; Closed-loop model; Polysome; PABP(PABPC1)
Categories
Funding
- National Research Founda-tion of Korea - Ministry of Science and ICT [NRF-2015R1A3A2033665, 2020R1A2C1012345]
- National Research Foundation of Korea [2020R1A2C1012345] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
Eukaryotic translation is a complex process involving various translation factors. A study found that the co-localization of eIF4E, eIF4G, and PABP proteins on mRNA is minimal, suggesting that the communication between them during translation may be transient.
Eukaryotic translation is a complex process that involves the interplay of various translation factors to convert genetic information into a specific amino acid chain. According to an elegant model of eukaryotic translation initiation, the 3' poly(A) tail of an mRNA, which is occupied by poly(A)-binding proteins (PABPs), communicates with the 5'-cap bound by eIF4E to enhance translation. Although the circulari-zation of mRNA resulting from the communication is widely understood, it has yet to be directly observed. To explore mRNA circularization in translation, we analyzed the level of colocalization of eIF4E, eIF4G, and PABP on individual mRNAs in polysomal and subpolysomal fractions using single polysome analysis. Our results show that the three tested proteins barely coexist in mRNA in either polysomal or subpolysomal fractions, implying that the closed-loop structure generated by the communication be-tween eIF4E, eIF4G, and PAPB may be transient during translation. (C) 2022 The Authors. Published by Elsevier Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available