Microcella aerolata sp. nov., isolated from electronic waste-associated bioaerosols

A Gram-positive, non-motile, non-spore-forming and short rod-shaped actinomycete strain, designated GA224(T), was isolated from electronic waste-associated bioaerosols. The optimal growth conditions for this isolate, a facultatively anaerobic bacterium, were 37 degrees C and pH 8.0. The cell-wall peptidoglycan type was B2 gamma, with 2,4-diaminobutyric acid (DAB) as the diamino acids, while the major menaquinone was MK-12. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, unidentified glycolipids and an unidentified lipid. The major cellular fatty acids were anteiso-C-15:0 and iso-C-16:0. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GA224(T) fell within the genus Microcella. The draft genome of strain GA224(T) comprised 2,495,189 bp with a G + C content of 72.2 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain GA224(T) and the type strain of the type species of Microcella species were lower than 95% and 70%, respectively. Based on the phenotypic, chemotaxonomic and genomic data, strain GA224(T) represents a novel species, for which the name Microcella aerolata sp. nov. is proposed, with GA224(T) as the type strain (= GDMCC 1.2165( T) = JCM 34462( T)).

Automated summary

A new Gram-positive short rod-shaped actinomycete strain GA224(T) was isolated from electronic waste-associated bioaerosols. The strain was a facultative anaerobic bacterium with specific cell-wall peptidoglycan, menaquinone, lipid profile, and fatty acid composition. Phylogenetic analysis and genomic comparison confirmed its classification within the Microcella genus as a novel species, Microcella aerolata.