Topographic attributes override impacts of agronomic practices on prokaryotic community structure

While topography can infer erosion potential, the practice of conventional agronomic management can trigger accelerated erosion and pose major threats to soil assets such as biodiversity. The majority of farmlands in Upper-Eastern Ghana are moderately hilly and highly susceptible to erosion. This study pioneered the comparative and interactive effects of topography and conventional versus conservation agriculture practices (reduced tillage, main crop and cover crop, crop residue retention vs. removal) in treatments amended with 0, 40, and 80 kg ha(-1 )N on soil physicochemical properties and microbiota. Topography imposed profound shifts in soil physiochemical properties and prokaryotic community structure. Foot-slope soils harbored higher prokaryotic rich-ness and diversity compared to the up-slope. Bacillaceae (28.95%) and anaerobic bacteria increased in relative abundance in foot-slope soils, while Micrococcaceae (25.79%) gained prominence in up-slope soils. The effect of tillage was significant in foot-slope while crop rotation was influential in up-slope soils on structuring the pro-karyotic community. The interactive effect of slope x tillage was significant in altering soil physiochemical properties, but not prokaryotic community structure. Variation in prokaryotic community composition was explained by soil physiochemical properties (14.5%), elevation as a proxy for topography (11.3%), and spatial distance (10.8%), but rather weakly overall by agronomic practices. Among the soil physicochemical properties, pH, clay content, total C%, volumetric water content, temperature, cation exchange capacity, and NO3--N were relevant factors influencing the soil microbiota. Geomorphic and soil edaphic properties appeared to interact and were the primary triggers of variation in soil microbiota and their responses to the range of agronomic practices that incorporated conservation management outcomes.

Automated summary

This study focused on the comparative and interactive effects of topography and conventional versus conservation agriculture practices on soil physicochemical properties and microbiota. The results showed that topography had a profound influence on soil properties and microbial communities. Different tillage and crop rotation practices had different effects on prokaryotic community structure in different topographic conditions. Soil physicochemical properties, elevation, and spatial distance played important roles in shaping the microbial composition.