A Continuum-Tensegrity Computational Model for Chondrocyte Biomechanics in AFM Indentation and Micropipette Aspiration

Mechanical stimuli are fundamental in the development of organs and tissues, their growth, regeneration or disease. They influence the biochemical signals produced by the cells, and, consequently, the development and spreading of a disease. Moreover, tumour cells are usually characterized by a decrease in the cell mechanical properties that may be directly linked to their metastatic potential. Thus, recently, the experimental and computational study of cell biomechanics is facing a growing interest. Various experimental approaches have been implemented to describe the passive response of cells; however, cell variability and complex experimental procedures may affect the obtained mechanical properties. For this reason, in-silico computational models have been developed through the years, to overcome such limitations, while proposing valuable tools to understand cell mechanical behaviour. This being the case, we propose a combined continuous-tensegrity finite element (FE) model to analyse the mechanical response of a cell and its subcomponents, observing how every part contributes to the overall mechanical behaviour. We modelled both Atomic Force Microscopy (AFM) indentation and micropipette aspiration techniques, as common mechanical tests for cells and elucidated also the role of cell cytoplasm and cytoskeleton in the global cell mechanical response.

Automated summary

Mechanical stimuli play a fundamental role in the development and disease of organs and tissues. The study of cell biomechanics is gaining increasing interest, with experimental and computational models being used to understand cell mechanical behavior. This study proposes a combined continuous-tensegrity finite element (FE) model to analyze the mechanical response of cells and their subcomponents.