Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 61, Issue 41, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202207188
Keywords
Activity-Based Probes; Bio-Imaging; Bio-Molecular Assays; Forster Resonance Energy Transfer (FRET); Quenchers
Categories
Funding
- National Key R&D Program of China [2020YFA0709900]
- National Natural Science Foundation of China [22077101, 22004099]
- Department of Science & Technology of Shaanxi Province [2020GXLH-Z-008, 2020GXLH-Z-021, 2020GXLH-Z-023]
- Northwestern Polytechnical University [2020GXLH-Z-008, 2020GXLH-Z-021, 2020GXLH-Z-023]
- Natural Science Foundation of Shaanxi Province [2022JM-130]
- Open Project Program of Wuhan National Laboratory for Optoelectronics [2020WNLOKF023]
- Key Research and Development Program of Shaanxi [2020ZDLGY13-04]
- Fundamental Research Funds for the Central Universities
- Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University [CX2022034]
- Open Project Program of the Analytical & Testing Center of Northwestern Polytechnical University [2020T018]
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This article summarizes the advances of small-molecule quenchers used in FRET-based probes, and provides suggestions for the rational design and selection of fluorophore-quencher FRET pairs that are compatible with challenging analytical applications in various biological systems.
For fluorogenic probes, Forster resonance energy transfer (FRET) is one of the most widely used mechanisms to realize the detection of biochemical activities. Dark quenchers relax from the excited state to the ground state non-radiatively, and are promising alternatives to fluorescent FRET acceptors. Small-molecule (dark) quenchers have been widely used as acceptors in FRET-based probes to monitor various physiological processes with minimum background signal. Herein, we summarize the relevant advances of small-molecule quenchers that are used in FRET-based probes. This Review is intended to provide suggestions regarding the rationale design and selection of appropriate fluorophore-quencher FRET pairs, which are fully compatible with challenging analytical applications in various biological systems. Finally, an outlook of the future biomedical applications and developments of this field is presented.
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