Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 61, Issue 42, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202209469
Keywords
Biosensors; Cell Adhesion; Drug Discovery; Membrane Proteins; Surface Plasmon Resonance
Categories
Funding
- National Institute of General Medical Sciences of the National Institutes of Health grant [R01GM107165]
- NSF program [NNCI-ECCS-1542160]
Ask authors/readers for more resources
Plasmonic scattering microscopy (PSM) adds a dry objective to a surface plasmon resonance (SPR) system, providing higher spatial resolution and larger field of view. PSM allows studying multiple cells simultaneously and quantifying the mechanical properties of cell-substrate contacts.
Surface plasmon resonance microscopy (SPRM) is an excellent platform for in situ studying cell-substrate interactions. However, SPRM suffers from poor spatial resolution and small field of view. Herein, we demonstrate plasmonic scattering microscopy (PSM) by adding a dry objective on a popular prism-coupled surface plasmon resonance (SPR) system. PSM not only retains SPRM's high sensitivity and real-time analysis capability, but also provides approximate to 7 times higher spatial resolution and approximate to 70 times larger field of view than the typical SPRM, thus providing more details about membrane protein response to ligand binding on over 100 cells simultaneously. In addition, PSM allows quantifying the target movements in the axial direction with a high spatial resolution, thus allowing mapping adhesion spring constants for quantitatively describing the mechanical properties of the cell-substrate contacts. This work may offer a powerful and cost-effective strategy for upgrading current SPR products.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available