Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 61, Issue 40, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202207971
Keywords
Biocatalysis; Photocatalysis; Redox Chemistry; Reductions; Transmembrane Shuttling
Categories
Funding
- European Union's Horizon 2020 research and innovation program under the Marie Skodowska-Curie grant [764920]
- Austrian Science Fund (FWF) [DOC 46-B21]
- BMK
- BMDW
- SFG
- Standortagentur Tirol
- Government of Lower Austria und Vienna Business Agency
- University of Graz
- Field of Excellence BioHealth
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A transmembrane electron shuttling system propelled by cyanobacterial photosynthesis was established to drive extracellular NAD(P)H-dependent redox reactions, achieving high conversions and optical purity of products.
Many biocatalytic redox reactions depend on the cofactor NAD(P)H, which may be provided by dedicated recycling systems. Exploiting light and water for NADPH-regeneration as it is performed, e.g. by cyanobacteria, is conceptually very appealing due to its high atom economy. However, the current use of cyanobacteria is limited, e.g. by challenging and time-consuming heterologous enzyme expression in cyanobacteria as well as limitations of substrate or product transport through the cell wall. Here we establish a transmembrane electron shuttling system propelled by the cyanobacterial photosynthesis to drive extracellular NAD(P)H-dependent redox reactions. The modular photo-electron shuttling (MPS) overcomes the need for cloning and problems associated with enzyme- or substrate-toxicity and substrate uptake. The MPS was demonstrated on four classes of enzymes with 19 enzymes and various types of substrates, reaching conversions of up to 99 % and giving products with >99 % optical purity.
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