4.8 Article

Vanadium Disulfide Nanosheet Boosts Optical Signal Brightness as a Superior Enzyme Label to Improve the Sensitivity of Lateral Flow Immunoassay

Journal

ANALYTICAL CHEMISTRY
Volume 94, Issue 24, Pages 8693-8703

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c01008

Keywords

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Funding

  1. National Natural Science Foundation of China [31972150]
  2. Key Industrial Innovation Chain Project of Shaanxi Province [2019ZDLSF07-08]
  3. Shaanxi Provincial Science Fund for Distinguished Young Scholars [2018JC-011]
  4. Natural Science Foundation Project of Guangdong Provincial [2020A1515010778]

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In this study, vanadium disulfide nanosheet (VS2NS) was successfully applied as an enzyme label in lateral flow immunoassay (LFIA) for the detection of 17-β-estradiol. Compared to natural horseradish peroxidase, VS2NS exhibited better catalytic performance, stability, and adsorption ability, resulting in lower detection limit and wider linear range in the LFIA.
The color-enzyme lateral flow immunoassay (LFIA) has attracted widespread attention to expand the detection range and improve sensitivity via amplifying the color signal after catalyzing the substrate. As a kind of layered transition-metal dichalcogenide ( TMD), the vanadium disulfide nanosheet (VS2NS) possesses superior peroxidase-like catalytic activity. Here, a VS2NS was applied as an enzyme label in the LFIA to detect 17 ss-estradiol (E2). Compared to natural horseradish peroxidase, the VS2NS expresses a more prominent enzyme catalytic performance, stability, and adsorption ability. Under optimal conditions, the calculated limit of detection (cLOD) of the VS2NS-based LFIA is 0.065 ng mL(- 1) for E2, which is sixfold lower than that of the optimized colloidal nanoparticle-based LFIA (cLOD = 0.406 ng mL(-1))(.) Besides, the detection linear range of the VS2NS-based LFIA can be widened by 1.5 times after the catalytic reaction. Moreover, the VS2NS-based LFIA exhibits excellent practicability in real sample detection. Simultaneously, this study helps open up the application of the VS2NS in the trace analysis of LFIAs, which can broaden TMDs' scope of application and better show their properties of color enzymes.

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