Journal
ANALYTICA CHIMICA ACTA
Volume 1220, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aca.2022.340065
Keywords
Liquid crystal; Aqueous microdroplets; Surfactant; Rolling circle amplification; Colorimetric assay; AFB1
Categories
Funding
- Natural Science Foundation of Shan-dong Province [ZR2020QB153]
- National Natural Science Foundation of China [62105175]
- Taishan Scholars Program [ts201712063]
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A colorimetric liquid crystal-based assay is reported for ultrasensitive detection of the group I carcinogen AFB1. Detection of AFB1 is achieved through specific recognition and rolling circle amplification reaction. The developed method shows good linear relationship and low detection limit.
The detection of AFB1 that is a group I carcinogen is significantly important for food safety. Herein, we report a colorimetric liquid crystal (LC)-based assay that allows the ultrasensitive detection of AFB1. When an aqueous solution of a cationic surfactant is transferred onto the LCs dispersed with the aqueous microdroplets containing the anionic surfactants and horseperoxidase (HRP), it triggers the release of HRP due to the interfacial charge interaction. Because HRP can catalyze the colorless 3,3 '-5,5 '-tetramethylbenzidine (TMB) into yellow products, the response of the LCs dispersed with the aqueous microdroplets to the cationic surfactant is visually determined. In the presence of AFB1, the rolling circle amplification on magnetic beads (MBs) is triggered due to the specific recognition of AFB1 by its aptamer, which results in the generation of long chain single-stranded DNA on MBs. As the cationic surfactants are captured by the negatively charged ssDNA, it prevents the release of HRP into the aqueous solution. In contrast, in the absence of AFB1, HRP is released into the aqueous solution. The developed AFB1 sensing assay shows very good linear relationship with the detection limit of AFB1 determined
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