BIOACTIVITY OF EXTRACTS OF EUROPEAN LAMIUM SPECIES ON CYTOKINE SECRETION FROM HUMAN NEUTROPHILS AND PERIPHERAL BLOOD MONONUCLEAR CELLS

Some representative species of the Lamium genus, such as L. album, are used as traditional medicines in female inflammatory disorders. Despite their prerequisites for therapeutic relevance to human health, the potential effect of Lamium species on inflammation processing and the molecular understanding of the attributes of most Lamium species for health aspects along with covering the detailed quality requirements has not been elucidated. The aim of the study was a complex qualitative and quantitative (UHPLC-DAD-MSn, HPLC-DAD) insight into the chemical composition of aqueous-methanolic (50%, v/v) extracts prepared from herbs of Lamium such as L. album (LA), L. amplexicaule (LAM), L. galeobdolon (LG), L. hybridum (LH), L. maculatum (LM), L. orvala (LO), and L. purpureum (LP) collected in different regions of Poland and Germany. The biological investigations of selected representative extracts (5-100 mu g/mL) included a screening of cytokines (tumor necrosis factor (TNF)-alpha, interleukins (IL)-1 beta, IL-8, IL-6, IL-10) secretion in LPS-stimulated human neutrophils (PMN) and peripheral blood mononuclear cells (PBMC). The prevailing compounds of Lamium herbs' extracts were iridoids (lamalbid), phenylpropanoids (verbascoside), flavonoids (rutin), and depsides (chlorogenic acid). The extracts significantly inhibited TNF-alpha secretion both in neutrophils (8.5-58.1%) and peripheral blood mononuclear cells (5.8-73.7%). In conclusion, the chemical diversity within Lamium was observed particularly in the case of lamalbid, rutin, and chlorogenic acid content. The inhibition of cytokine release, in particular TNF-alpha, by some extracts in in vivo-relevant models indicates their potential role in inflammation resolution.

Automated summary

This study provides a comprehensive analysis of the chemical composition of Lamium species and demonstrates their inhibitory effects on cytokine secretion. The main compounds found in Lamium herbs' extracts are lamalbid, rutin, and chlorogenic acid. These findings suggest the potential role of Lamium species in inflammation resolution.