Size- and Ligand-Dependent Transport of Nanoparticles in Matricaria chamomilla as Demonstrated by Mass Spectroscopy and X-ray Fluorescence Imaging

Matricaria chamomilla flowers were incubated with gold nanoparticles of different sizes ranging from 1.4 to 94 nm. After different incubation times of 6, 12, 24, and 48 h, the gold distribution in the flowers was destructively measured by inductively coupled plasma mass spectrometry (ICP-MS) and non-destructively measured by X-ray fluorescence imaging (XFI) with high lateral resolution. As a control, the biodistribution of iodine ions or iodine-containing organic molecules (iohexol) was determined, in order to demonstrate the feasibility of mapping the distribution of several elements in parallel. The results show a clear size-dependent transport of the nanoparticles. In addition, the surface chemistry also plays a decisive role in disposition. Only the 1.6 nm nanoparticles coated with acetylcysteine could be efficiently transported through the stem of the flowers into the petals. In this case, almost 80% of the nanoparticles which were found within each flower were located in the petals. The study also highlights the potential of XFI for in situ recording of in vivo analyte biodistribution.

Automated summary

This study incubated Matricaria chamomilla flowers with gold nanoparticles of different sizes and measured the gold distribution. The results show that the transport of nanoparticles is size-dependent and the surface chemistry also plays a role. The nanoparticles coated with acetylcysteine could be efficiently transported into the petals.