Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 54, Issue 3, Pages 676-686Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02731-15
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Funding
- NARO
- Alliance Biosecure Foundation under the aegis of the Fondation pour la Recherche Medicale [FABS 201401]
- Intramural Research Program of the National Institute of Allergy and Infectious Diseases (DIR, NIAID)
- Intramural Research Program of the NIAID
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Prion diseases of cattle include the classical bovine spongiform encephalopathy (C-BSE) and the atypical H-type BSE (H-BSE) and L-type BSE (L-BSE) strains. Although the C-and L-BSE strains can be detected and discriminated by ultrasensitive real-time quaking-induced conversion (RT-QuIC) assays, no such test has yet been described for the detection of H-BSE or the discrimination of each of the major bovine prion strains. Here, we demonstrate an RT-QuIC assay for H-BSE that can detect as little as 10(-9) dilutions of brain tissue and neat cerebrospinal fluid samples from clinically affected cattle. Moreover, comparisons of the reactivities with different recombinant prion protein substrates and/or immunoblot band profiles of proteinase K-treated RT-QuIC reaction products indicated that H-, L-, and C-BSE have distinctive prion seeding activities and can be discriminated by RT-QuIC on this basis.
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