Journal
ADVANCED BIOLOGY
Volume 6, Issue 6, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adbi.202101317
Keywords
exosomes; extracellular vesicles; mesenchymal stem cells; metabolomics; neural differentiation; neural stem cells; subventricular zone
Categories
Funding
- Scientific and Technological Research Council of Turkey (TUBITAK) [116S476]
Ask authors/readers for more resources
This study demonstrates that rat subventricular zone neural stem cell-derived exosomes can control the neural-lineage specification of human mesenchymal stem cells. Through miRNA analysis and multi-omics analysis, the ability to use these exosomes to induce hMSCs into a neuroglial or neural stem cell phenotype and genotype is demonstrated. This research presents a new strategy that harnesses the molecular content of exosomes to modulate the fate of stem cells.
Extracellular nanovesicles, particularly exosomes, can deliver their diverse bioactive biomolecular content, including miRNAs, proteins, and lipids, thus providing a context for investigating the capability of exosomes to induce stem cells toward lineage-specific cells and tissue regeneration. In this study, it is demonstrated that rat subventricular zone neural stem cell-derived exosomes (rSVZ-NSCExo) can control neural-lineage specification of human mesenchymal stem cells (hMSCs). Microarray analysis shows that the miRNA content of rSVZ-NSCExo is a faithful representation of rSVZ tissue. Through immunocytochemistry, gene expression, and multi-omics analyses, the capability to use rSVZ-NSCExo to induce hMSCs into a neuroglial or neural stem cell phenotype and genotype in a temporal and dose-dependent manner via multiple signaling pathways is demonstrated. The current study presents a new and innovative strategy to modulate hMSCs fate by harnessing the molecular content of exosomes, thus suggesting future opportunities for rSVZ-NSCExo in nerve tissue regeneration.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available