Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1038, Issue -, Pages 1-11Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2016.10.014
Keywords
Panax ginseng; Component analysis; Drug screening; Target cell extraction; UPLC-QTOF-MS; SH-SY5Y cell
Funding
- National Natural Science Foundation of China [81260605, 81560648]
- National Institutes of Health [AT004418, AT005362]
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Ginseng is one of the most widely used natural medicines in the world. Recent studies have suggested Panax ginseng has a wide range of beneficial effects on aging, central nervous system disorders, and neurodegenerative diseases. However, knowledge about the specific bioactive components of ginseng is still limited. This work aimed to screen for the bioactive components in Panax ginseng that act against neurodegenerative diseases, using the target cell-based bioactivity screening method. Firstly, component analysis of Panax ginseng extracts was performed by UPLC-QTOF-MS, and a total of 54 compounds in white ginseng were characterized and identified according to the retention behaviors, accurate MW, MS characteristics, parent nucleus, aglycones, side chains, and literature data. Then target cell-based bioactivity screening method was developed to predict the candidate compounds in ginseng with SHSY5Y cells. Four ginsenosides, Rg2, Rhi, Ro, and Rd, were observed to be active. The target cell-based bioactivity screening method coupled with UPLC-QTOF-MS technique has suitable sensitivity and it can be used as a screening tool for low content bioactive constituents in natural products. (C) 2016 Elsevier B.V. All rights reserved.
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