Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1014, Issue -, Pages 70-74Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2016.01.052
Keywords
Saliva; Clinical analysis; Inorganic anions; Field amplified sample injection; Capillary electrophoresis; Capacitively coupled contactless; conductivity detection
Funding
- Natural Science Foundation of China [21205042]
- Daxia Foundation of East China Normal University [2014DX-418]
- Students Innovative Experimental Project of Shanghai Municipality [201510269082]
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A capillary electrophoresis approach with capacitively coupled contactless conductivity detection method has been developed for the determination of inorganic metabolites (thiocyanate, nitrite and nitrate) in human saliva. Field amplified sample injection, as a simple sample stacking technique, was used in conjunction for online preconcentration of above inorganic anions. A selective separation for the target anions from other coexisting constituents present in saliva could be obtained within 14 min in a 10 mmol/L His-90 mmol/L HAc buffer (pH 3.70) at the separation voltage of -18 kV. The limits of detection and limits of quantification of the three analytes were within the range of 3.1-4.9 ng/mL (S/N = 3) and 10-16 ng/mL (S/N = 10), respectively. The average recovery data were in the range of 81-108% at three different concentrations. This method provides a simple, rapid and direct approach for metabolite analyses of nitric oxide and cyanide based on noninvasive saliva sample, which presents a potential fast screening tool for clinical test. (C) 2016 Elsevier B.V. All rights reserved.
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