Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1466, Issue -, Pages 96-104Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2016.09.001
Keywords
Whelk-01 superficially porous chiral; stationary phase; Ultrafast enantioseparations; Mass transfer kinetics; Sub-second separation; Normal phase mode
Funding
- Italian University and Scientific Research Ministry [PRIN 2012ATMNJ_003]
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Pirkle-type Whelk-01 chiral stationary phase (CSP) was prepared on 2.6 mu m superficially porous particles (SPPs). The chromatographic behavior of columns packed with this new CSP was compared with that of columns packed respectively with 1.8 and 2.5 mu m Whelk-01 fully porous particles (FPPs). In the comparison, both thermodynamic and kinetic aspects were considered. Contrary to initial expectations, chiral columns packed with 2.6 mu m SPPs were quasi-comparable to those packed with 2.5 mu m FPPs, apparently due to larger contributions to band broadening from both eddy dispersion and, especially for the second eluted enantiomer, adsorption desorption kinetics. These findings raise the question if SPPs, in spite of the undeniable advantages of their morphology to speed up mass transfer, are always the best choice for high-efficient ultrafast chiral separations. The last part of the work focuses on the use of short columns (10 mm long) and very high flow rates to realize the separation of the enantiomers of trans-stilbene oxide (TSO) in normal phase mode in less than 1 s. (C) 2016 Elsevier B.V. All rights reserved.
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