Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1467, Issue -, Pages 356-362Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2016.07.061
Keywords
HPLC; Chiral selector; Sulfobutylether-beta-cyclodextrin; Dynamic coating; Mobile phase additive
Funding
- Grant Agency of Charles University [790314]
- Czech Science Foundation [P206/14-19278P, 16-05942S]
Ask authors/readers for more resources
Various amino acids, dipeptides and their isomers were (enantio)separated using sulfobutylether-beta-cyclodextrin as a chiral selector. Two different approaches were employed: first, dynamic coating of sulfobutylether-beta-cyclodextrin onto a strong anion-exchange stationary phase and, second, use of sulfobutylether-beta-cyclodextrin as a mobile phase additive in a separation system with a C18 column. Measurements were carried out using RP-HPLC and hydrophilic interaction liquid chromatography. Mobile phases composed of organic modifier (methanol) and four different aqueous parts: (i) deionized water, (ii) an aqueous solution of formic acid (pH 2.1), (iii) ammonium acetate buffer(pH 4.7), and (iv) ammonium acetate buffer (pH 8.8) in various volume ratios. Under these separation conditions, out of 23 chiral analytes, 9 were baseline enantio-resolved and 7 were partially separated. Of 9 mixtures of dipeptide isomers, 8 were baseline-separated. Sulfobutylether-beta-cyclodextrin proved to be suitable for the separation of chiral and also achiral analytes. The use of sulfobutylether-beta-cyclodextrin as a dynamic coating agent or as a mobile phase additive depends on the particular chromatographic system and analytes of interest. (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available