Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1450, Issue -, Pages 29-37Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2016.04.061
Keywords
Pareto-optimality; interpretive optimization; LC x LC; method development; synthetic dyes; retention prediction
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The challenge of fully optimizing LC x LC separations is horrendous. Yet, it is essential to address this challenge if sophisticated LC x LC instruments are to be utilized to their full potential in an efficient manner. Currently, lengthy method development is a major obstacle to the proliferation of the technique, especially in industry. A program was developed for the rigorous optimization of LC x LC separations, using gradient-elution in both dimensions. The program establishes two linear retention models (one for each dimension) based on just two LC x LC experiments. It predicts LC x LC chromatograms using a simple van-Deemter model to generalize band-broadening. Various objectives (analysis time, resolution, orthogonality) can be implemented in a Pareto-optimization framework to establish the optimal conditions. The program was successfully applied to a separation of a complex mixture of 54 aged, authentic synthetic dyestuffs, separated by ion-exchange chromatography and ion pair chromatography. The main limitation experienced was the retention-time stability in the first (ion-exchange) dimension. Using the PIOTR program LC x LC method development can be greatly accelerated, typically from a few months to a few days. (C) 2016 Elsevier B.V. All rights reserved.
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