Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1437, Issue -, Pages 49-57Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2016.02.017
Keywords
Dispersive liquid-liquid microextraction; Microwave-assisted derivatization; Mass spectrometry sensitization; 20(S)-protopanaxadiol; 20(S)-protopanaxatriol; Pharmacokinetics
Funding
- National Natural Science Foundation of China [81303179, 21575007, 21405093, 21405094, 31301595, 81573574, 21505084, 21475074, 21475075, 21305076]
- Natural Science Foundation of Shandong Province [ZR2013BQ018, ZR20138Q019, ZR20148Q009, ZR2014BM029, ZR2015CM040]
- Open Funds of the State Key Laboratory of Electroanalytical Chemistry [SKLEAC201506]
- Experimental Research Project of Qufu Normal University [sj201402]
- Foundation of Qufu Normal University [BSQD2012019, 2012023]
Ask authors/readers for more resources
This paper, for the first time, reported a speedy hyphenated technique of low toxic dual ultrasonic assisted dispersive liquid liquid microextraction (dual-UADLLME) coupled with microwave-assisted derivatization (MAD) for the simultaneous determination of 20(S)-protopanaxadiol (PPD) and 20(S)-protopanaxatriol (PPT). The developed method was based on ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) detection using multiple-reaction monitoring (MRM) mode. A mass spectrometry sensitizing reagent, 4'-carboxy-substituted rosamine (CSR) with high reaction activity and ionization efficiency was synthesized and firstly used as derivatization reagent. Parameters of dual-UADLLME, MAD and UHPLC-MS/MS conditions were all optimized in detail. Low toxic brominated solvents were used as extractant instead of traditional chlorinated solvents. Satisfactory linearity, recovery, repeatability, accuracy and precision, absence of matrix effect and extremely low limits of detection (LODs, 0.010 and 0.015 ng/mL for PPD and PPT, respectively) were achieved. The main advantages were rapid, sensitive and environmentally friendly, and exhibited high selectivity, accuracy and good matrix effect results. The proposed method was successfully applied to pharmacokinetics of PPD and PPT in rat plasma. (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available