Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1428, Issue -, Pages 185-192Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2015.07.032
Keywords
Hydrophilic interaction; Reversed-phase; Liquid chromatography-tandem mass spectrometry; Peptides; Antioxidant; Peach seed
Funding
- Ministry of Economy and Competitiveness [AGL2012-36362]
- Comunidad Autonoma of Madrid (Spain)
- FEDER program [S2013/ABI-3028]
- University of Alcala [CCG2013/EXP-028]
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HILIC- and RP-HPLC-ESI-Q-TOF identification of bioactive peptides with antioxidant capacity in peach by-products was carried out. Peach seeds contain more than 40% of proteins (as dried and defatted basis) and could constitute a cheap source of bioactive peptides. Extracted proteins were digested using four different commercial enzymes. Five assays based on different antioxidant mechanisms were employed for a reliable evaluation of the antioxidant capacity of the extracts. Thermolysin enzyme originated the extract with the most favorable antioxidant capacity. Probably due to a synergic effect among antioxidant peptides, it was not possible to find a peptide fraction with a higher antioxidant capacity than the whole extract. Eighteen peptides were identified in the whole hydrolysate when combining HILIC- and RP-HPLC-ESI-Q-TOF. A high percentage of hydrophobic amino acids were observed within their sequences which is a characteristic feature of the antioxidant nature of peptides. (C) 2015 Elsevier B.V. All rights reserved.
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