Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1448, Issue -, Pages 121-126Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2016.04.063
Keywords
Membrane adsorber; Membrane chromatography; Ion-exchange; Hydrophobic interaction; Affinity membrane adsorber
Funding
- National High Technology Research and Development Program of China (863 Program) [2014AA021006]
- 100 Talents Program of Chinese Academy of Sciences
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Polydopamine, as an intermediate layer coated on PES membrane, was applied to fabricate various membrane adsorbers. Anion-exchange, hydrophobic interaction and affinity membrane adsorbers prepared by this facile method exhibited a high selectivity in fractionation of IgG (immunoglobulin)/HSA (human serum albumin) mixture. The anion-exchange membrane adsorber containing polyethylenimine (PEI) improved the HSA purity from 17.7% to 96.7%; The hydrophobic interaction membrane adsorber with Dodecyl mercaptan (DDM) as ligand obtained an IgG purity of 94.6%; Histidine attached affinity membrane chromatography achieved nearly a 100% purity of IgG. The present work indicated that the polydopamine layer not only activated membrane surface to attach various adsorptive ligands under the mild condition, but also reduced non-specific adsorption. Due to the versatile conjunction function, this facile mussel-inspired coating is also promising for the preparation of diverse membrane adsorbers. (C) 2016 Elsevier B.V. All rights reserved.
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