The intrinsically photosensitive retinal ganglion cell (ipRGC) mediated pupil response in young adult humans with refractive errors

Purpose: The intrinsically photosensitive retinal ganglion cells (ipRGCs) signal environmental light, with axons projected to the midbrain that control pupil size and circadian rhythms. Post-illumination pupil response (PIPR), a sustained pupil constriction after short-wavelength light stimulation, is an indirect measure of ipRGC activity. Here, we measured the PIPR in young adults with various refractive errors using a custom-made optical system. Methods: PIPR was measured on myopic (-3.50 +/- 1.82 D, n = 20) and non-myopic (+0.28 +/- 0.23 D, n = 19) participants (mean age, 23.36 +/- 3.06 years). The right eye was dilated and presented with long-wavelength (red, 625 nm, 3.68 x 10(14) photons/cm2/s) and short-wavelength (blue, 470 nm, 3.24 x 10(14) photons/cm(2)/s) 1 s and 5 s pulses of light, and the consensual response was measured in the left eye for 60 s following light offset. The 6 s and 30 s PIPR and early and late area under the curve (AUC) for 1 and 5 s stimuli were calculated. Results: For most subjects, the 6 s and 30 s PIPR were significantly lower (p < 0.001), and the early and late AUC were significantly larger for 1 s blue light compared to red light (p < 0.001), suggesting a strong ipRGC response. The 5 s blue stimulation induced a slightly stronger melanopsin response, compared to 1 s stimulation with the same wavelength. However, none of the PIPR metrics were different between myopes and non-myopes for either stimulus duration (p > 0.05). Conclusions: We confirm previous research that there is no effect of refractive error on the PIPR. (c) 2020 Spanish General Council of Optometry. Published by Elsevier Espana, S.L.U.

Automated summary

This study measured the post-illumination pupil response (PIPR) in young adults with different refractive errors using a custom-made optical system. The results showed that refractive error did not have an effect on PIPR, but there was a significant impact of different wavelengths of light stimulation on PIPR.