Journal
JOURNAL OF CHEMOTHERAPY
Volume 29, Issue 3, Pages 144-149Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/1120009X.2016.1199506
Keywords
Pseudomonas aeruginosa; Carbapenem resistance; Carba NP test; Modified Hodge test; Carbapenem inactivation method
Funding
- Immunology Research Center of Tabriz University of Medical Sciences
- Tabriz University of Medical Sciences, Tabriz, Iran [5.47.2897]
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This investigation was undertaken to compare phenotypic and molecular methods for detection of carbapenemaseproducing Pseudomonas aeruginosa. A total of 245 non-duplicated isolates of P. aeruginosa were collected from hospitalized patients. Disc diffusion method was used to identify carbapenem-resistant bacteria. Three phenotypic methods, including Modified Hodge Test (MHT), Modified Carba NP (MCNP) test and Carbapenem Inactivation Method (CIM) were used for investigation of carbapenemase production. In addition, polymerase chain reaction (PCR) was used to detect carbapenemase encoding genes. Of 245 P. aeruginosa isolates investigated, 121 isolates were carbapenem-resistant. Among carbapenem-resistant isolates, 40, 39 and 35 isolates exhibited positive results using MHT, MCNP test and CIM, respectively. PCR indicated the presence of carbapenemase genes in 35 of carbapenem-resistant isolates. MHT showed low sensitivity and specificity for carbapenemase detection among P. aeruginosa isolates in comparison to PCR. CIM was most affordable and highly specific than MCNP test compared with the molecular method.
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