Quantitative proteomic dataset of mouse caput epididymal epithelial cells exposed to acrylamide in vivo

This article reports the proteomic legacy of in vivo exposure to the xenobiotic, acrylamide, on the epithelial cell population of the proximal segments of the mouse epididymis. Specifically, adult male mice were administered acrylamide (25 mg/kg bw/day) or vehicle control for five consecutive days before dissection of the epididymis. Epididymal epithelial cells were isolated from the proximal (caput) epididymal segment and subjected to quantitative proteomic analysis using multiplexed tandem mass tag (TMT) labeling coupled to mass spectrometry. Here, we report the data generated by this strategy, including the identification of 4405 caput epididymal epithelial cell proteins, approximately 6.8% of which displayed altered expression in response to acrylamide challenge. Our interpretation and discussion of these data features in the article Acrylamide modulates the mouse epididymal proteome to drive alterations in the sperm small non-coding RNA profile and dysregulate embryo development (C) 2022 Published by Elsevier Inc.

Automated summary

This study reports the proteomic effects of in vivo exposure to acrylamide on the epithelial cell population of the mouse epididymis. The results show that acrylamide can alter the expression of proteins in the epididymal epithelial cells and impact the sperm small non-coding RNA profile and embryo development.