Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 25, Issue 45, Pages 7003-7016Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201502458
Keywords
-
Categories
Funding
- FIRB RENAME project [RBAP114AMK]
- PRIN project [2012SK7ASN]
Ask authors/readers for more resources
Peptide materials based on the aggregation of polyphenylalanine conjugates containing gadolinium complexes and acting as potential contrast agents (CAs) in magnetic resonance imaging (MRI) are described. Monomers contain two (F2) or four (F4) phenylalanine residues for self-assembly, a chelating agent, 1,4,7,10-tetraazacyclododecane-N, N, N, N -tetraacetic acid (DOTA) or diethylenetriaminepentaacetic acid (DTPA), for achieving gadolinium coordination, and ethoxylic linkers at two (L-2) or six (L-6) poly(ethylene glycol) (PEG) units between the chelating group and the peptide region. Both DOTA and DTPA tetraphenylalanine derivatives, and their gadolinium complexes DOTA(Gd)-L-6-F4 and DTPA(Gd)-L-6-F4, are able to self-aggregate at very low concentration. Structural characterization, obtained by circular dichroism and infrared measurements, confirms the amyloid type fibril formation in which an antiparallel peptide alignment is preferred. Amyloid type fibril formation is also observed, in solid state, by transmission electron microscopy images and X-ray diffraction patterns. The relaxivity values of DOTA(Gd)-L-6-F4 and DTPA(Gd)-L-6-F4 and their ability to enhance the MRI cellular response on the J774A. 1 mouse macrophages cell line indicate that these peptide materials are promising candidate as a new class of supramolecular gadolinium based MRI contrast agents.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available