Journal
FOOD CHEMISTRY-X
Volume 14, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.fochx.2022.100273
Keywords
Treponema primitia alpha 1-2-fucosyltransferase; One-pot multienzyme synthesis; Lacto-N-fucopentaose I; Anti-enterovirus 71
Categories
Funding
- Key Project of the Natural Science Foundation of Fujian Province [2020J02032]
- Fujian 'Young Eagle Program' Youth Top Talent Program
- Project of Excellent Master Degree Thesis and Double First-Class Con-struction Plan of Fujian Agriculture and Forestry University [KSYLX013]
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The translation abstract introduces a new enzyme, Tp2FT, which may have potential for synthesizing important fucosylated oligosaccharide LNFP I. The study found that LNFP I can reduce the concentrations of inflammatory cytokines and effectively inhibit the synthesis of enterovirus 71 proliferation, suggesting its potential therapeutic effect on hand foot mouth disease.
Fucosylated oligosaccharides have important biological functions as well as an excellent antiviral activity. A novel alpha 1-2-fucosyltransferase (alpha 2FT) from Treponema primitia (Tp2FT) was cloned and expressed in Escherichia coli BL21(DE3) and purified as an N-His(6)-tagged fusion protein (His(6)-Tp2FT). Mass spectrometry was carried out to identify the products of enzymatic reaction. The Tp2FT exhibited strict acceptor substrate specificity for type 1 structure (Gal beta 1-3GlcNAc)-containing glycans. It might be a promising emzyme for the chemo-enzymatic synthesis of lacto-N-fucopentaose I (LNFP I), which is one of the important fucosylated oligosaccharides. In this study, different in vitro experiments were used to study the biological activities of LNFP I. It could reduce the concentrations of inflammatory cytokines and effectively inhibit the synthesis of enterovirus 71 proliferation. LNFP I was an inhibitor of enterovirus 71 in the early stages of infection, it can used in infant nutrition and might provide a new drug for hand foot mouth disease.
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